|Spec container||:||Serum Gel|
|Volume required||:||5 ml|
|Reference range||:||<7.0 U/ml|
Methodology: Enzyme Immunoassay using human recombinant antigen.
Indications: As an aid in the diagnosis of gluten-sensitive enteropathies such as coeliac disease and dermatitis herpetiformis
Protocol: Samples are screened for IgA-specific anti-tissue transglutaminase (tTG). Positive tests are confirmed by IgA immunofluorescence using endomysium. Total IgA is measured to exclude IgA deficiency. If IgA deficiency is present then IgG-specific antibodies are performed by immunofluorescence using endomysium.
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|Anti-endomysial antibody demonstrated by immunofluorescence|