| (Immunology) |
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| Investigation | : | Anti-Tissue Transglutaminase | ||||||||||
| Spec container | : | Serum Gel | ||||||||||
| Volume required | : | 5 ml | ||||||||||
| Reference range | : | <7.0 U/ml | ||||||||||
| Turnaround | : | <3 days | ||||||||||
Methodology: Enzyme Immunoassay using human recombinant antigen.
Indications: As an aid in the diagnosis of gluten-sensitive enteropathies such as coeliac disease and dermatitis herpetiformis
Protocol: Samples are screened for IgA-specific anti-tissue transglutaminase (tTG). Positive tests are confirmed by IgA immunofluorescence using endomysium. Total IgA is measured to exclude IgA deficiency. If IgA deficiency is present then IgG-specific antibodies are performed by immunofluorescence using endomysium.
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Anti-endomysial antibody demonstrated by immunofluorescence |