DAT - Direct Antiglobulin Test
(Blood Transfusion)
Investigation : DAT - Direct Antiglobulin Test
Specimen type : K3E Crossmatch K3EDTA
Spec container : 6ml (PINK) 
Volume required : 1 full 6ml sample
Reference range : None
Turnaround : As required
Special sample requirements Fully labelled and not haemolysed. Sample and request must be handwritten.
Patient Preparation None
Storage Requirements The sample tube should be transferred to the laboratory for storage between 2-8°C.
Turnaround time As required
Clinical Application Direct Antiglobulin Test (DAT) is performed to demonstrate in vivo sensitisation of a patientís red cells, with incomplete (IgG) antibodies.

It is used for the detection and differential diagnosis of various forms of immune hemolysis including autoimmune hemolytic anemia, hemolytic transfusion reactions, drug-induced hemolysis, and hemolytic disease of the fetus newborn (HDFN).
Clinical Interpretation Reports as positive or negative.
CPA/UKAS Accredited LCL Transfusion laboratories are accredited to ISO 15189 by United Kingdom Accreditation Service (UKAS) for the clinical services they provide. They are also licensed by the Medicines and Healthcare products Regulatory Agency (MHRA) for the services that fall under the Blood Safety and Quality Regulations (BSQR).


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