HLA Typing
 (H & I)
Investigation : HLA Typing
Investigations HLA Typing:
Class I (HLA-A, B, C)
Class II (HLA-DRB1, DRB3/4/5, DQA1, DQB1, DPA1, DPB1)
Inform lab before sending Not usually required, unless samples are for deceased organ donor typing, or if the clinical urgency of the sample necessitates reduced testing times. Users are encouraged to contact the Consultant Clinical Scientist to discuss individual patient testing requirements.
Specimen type 4ml EDTA
Ideal time to take specimen Not applicable
Labelling requirements click here
How to take the specimen Whole blood into EDTA
Transport to the laboratory see H&I General Information Page
Test Times All transplant patients, regardless of whether renal or stem cell are required to have HLA typing performed on two separate samples bled on different dates in order to confirm identity and the accuracy of the HLA typing result. All donors intended to be used for transplant are also required to have confirmatory testing performed prior to proceeding to transplant.
Turnround 10 working days for the following:
  • HLA typing of renal transplant recipients
  • Renal patient related donors
  • Stem cell transplant recipients
  • Stem cell transplant related donors
  • Stem cell transplant unrelated donors

    6 hours from receipt of sample for deceased organ donors
    • Additional information HLA class I/II typing is undertaken by the H&I laboratory utilising a range of molecular typing methodologies, the choice of which is governed primarily by the degree of resolution clinically required.

    For renal transplant recipients:
  • HLA typing for HLA-A,B,DRB1 is performed as a minimum by PCR-SSOP (sequence specific oligonucleotide probes). Typing for additional loci (HLA-C, DQ, DP) is dependent upon the allo-sensitisation status of the patient.

    For renal transplant donors:
  • HLA typing for HLA-A,B,DRB1 is performed as a minimum by PCR-SSOP (sequence specific oligonucleotide probes). Typing for additional loci (HLA-C, DQ, DP) is dependent upon the allo-sensitisation status of the patient in order to define any potential donor specific alloreactivity.

    For Stem cell transplant patients:
  • HLA typing for HLA-A,B,DRB1 is performed as a minimum by PCR-SSOP. Where do familial donors are identified a donor may be sought via national and international donor registry searches. In these instances the patient will be HLA tested to the allelic resolution using next generation sequencing (NGS) methodology.

    For Stem cell transplant donors:
  • HLA typing for related donors is performed as a minimum by PCR-SSOP.
  • HLA typing for unrelated donor registry samples will be HLA tested to the allelic resolution using next generation sequencing (NGS) methodology.
    • Additional Examination Requests A primary sample must be received for this test




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