Transplant Immunology

SAMPLE REQUIREMENTS
TURNAROUND TIME

Investigation
Sample Requirements
Turnaround Time
Comments
HLA Class I & II Typing
Min 2.5ml EDTA

20ml EDTA required for patients with low white cells
10 Working days
15 days Confirmatory testing required for all recipients and donors prior to transplant.
HLA Class I (A,B,C,) Typing
Min 2.5ml EDTA

20ml EDTA required for patients with low white cells
10 Working days
Urgent samples by request.
HLA Class II (DR,DQ,DP)
Min 2.5ml EDTA

20ml EDTA required for patients with low white cells
10 Working days
Urgent samples by request.
High Resolution HLA Typing by NGS
Min 2.5ml EDTA

20ml EDTA required for patients with low white cells
4 Weeks
 
HLA B27 Status
Min 2.5ml EDTA
10 Working days
 
Crossmatch
Live related donor
Recipient- 10ml clotted
- 30ml EDTA
 
Donor- 60ml EDTA
5 Working days
Must be pre- booked with laboratory
Rituximab treatment interferes with test results. Please notify the laboratory if a recipient has received Rituximab.
Crossmatch
deceased donor
Recipient- 10ml clotted
- 30ml EDTA
 
Donor- Lymph node and spleen tissues
report to ward within 6 hours
Turnaround times monitored as a laboratory key performance indicator
Rituximab treatment interferes with test results. Please notify the laboratory if a recipient has received Rituximab.
HLA
antibody screen
Min 7ml serum
4 weeks
Urgent samples by request

Renal transplant patients require 3 monthly repeat testing
HLA antibody
HLA class I Identity
Min 7ml serum *
4 weeks
Urgent samples by request
HLA antibody
HLA class II Identity
Min 7ml serum *
4 weeks
Urgent samples by request
B*57:01 Testing
Min 2.5ml EDTA
7 Working days
Turnaround times monitored as a laboratory key performance indicator
Post HSCT monitoring (Chimerism analysis)
Up to 40ml EDTA
(depending on white cell count)
7 Working days
The specimen must not be refrigerated, it should be stored at room temperature and
delivered to the laboratory within 4hrs of collection.

Turnaround times monitored as a laboratory key performance indicator
HLA typing for disease associations (not B27 or B*57:01 testing)
2.5ml EDTA
10 Working days
Please specify the required HLA locus and disease on request card.
* HLA antibody identification is usually a reflexed test from the HLA-specific antibody screen