Investigation	Sample Requirements	Turnaround Time	Comments
HLA Class I & II Typing	Min 2.5ml EDTA 20ml EDTA required for patients with low white cells	10 Working days	15 days Confirmatory testing required for all recipients and donors prior to transplant. Please use the required EDTA anticoagulant do not use Lithium Heparin as it may affect the test performance
HLA Class I (A,B,C,) Typing	Min 2.5ml EDTA 20ml EDTA required for patients with low white cells	10 Working days	Urgent samples by request. Please use the required EDTA anticoagulant do not use Lithium Heparin as it may affect the test performance
HLA Class II (DR,DQ,DP)	Min 2.5ml EDTA 20ml EDTA required for patients with low white cells	10 Working days	Urgent samples by request. Please use the required EDTA anticoagulant do not use Lithium Heparin as it may affect the test performance
High Resolution HLA Typing by NGS	Min 2.5ml EDTA 20ml EDTA required for patients with low white cells	4 Weeks	Please use the required EDTA anticoagulant do not use Lithium Heparin as it may affect the test performance
HLA B27 Status	Min 2.5ml EDTA	10 Working days	Please use the required EDTA anticoagulant; do not use Lithium Heparin as it may affect the test performance
Crossmatch – Live related donor	Recipient - 10ml clotted - 30ml EDTA   Donor - 60ml EDTA	5 Working days	Must be pre- booked with laboratory Rituximab treatment interferes with test results. Please notify the laboratory if a recipient has received Rituximab.
Crossmatch – deceased donor	Recipient - 10ml clotted - 30ml EDTA   Donor - Lymph node and spleen tissues	report to ward within 6 hours	Turnaround times monitored as a laboratory key performance indicator Rituximab treatment interferes with test results. Please notify the laboratory if a recipient has received Rituximab.
HLA antibody screen	Min 7ml serum	4 weeks	Urgent samples by request Renal transplant patients require 3 monthly repeat testing
HLA antibody HLA class I Identity	Min 7ml serum *	4 weeks	Urgent samples by request
HLA antibody HLA class II Identity	Min 7ml serum *	4 weeks	Urgent samples by request
B*57:01 Testing	Min 2.5ml EDTA	7 Working days	Turnaround times monitored as a laboratory key performance indicator Please use the required EDTA anticoagulant do not use Lithium Heparin as it may affect the test performance
Post HSCT monitoring (Chimerism analysis)	Up to 40ml EDTA (depending on white cell count)	7 Working days	The specimen must not be refrigerated, it should be stored at room temperature and delivered to the laboratory within 4hrs of collection. Turnaround times monitored as a laboratory key performance indicator
HLA typing for disease associations (not B27 or B*57:01 testing)	2.5ml EDTA	10 Working days	Please specify the required HLA locus and disease on request card.

* HLA antibody identification is usually a reflexed test from the HLA-specific antibody screen