Human Immunodeficiency Virus (HIV)
(Microbiology)
Investigations Human Immunodeficiency Virus (HIV)
The virus causes depletion of CD4+ T cells and eventually lead to the development of opportunistic infection if left untreated. Human immunodeficiency virus type 1 accounts for the majority of HIV infections worldwide.

Serology Tests
HIV antibody/antigen screen (HIV-1/2 4th and 3rd generation and immunoblot tests)

Molecular Tests
HIV-1 RNA
HIV-1 Antiretroviral Resistance Genotype
HIV-1 Genotypic Tropism
HIV-1 Integrase
Inform lab before sending No, except for URGENT SAMPLES. Contact the laboratory on 0151 706 4410 to request urgent testing, including HIV-1 RNA.
Specimen type Serology
Whole blood: 5-10ml blood into a Serum (WHITE CAPPED) container.
EDTA: 10ml blood into a potassium EDTA (RED CAPPED) container.

Molecular
EDTA: 10ml blood into a potassium EDTA (RED CAPPED) container.
PBMC: 5-10ml blood into a potassium EDTA (RED CAPPED) container.
Washed Sperm: Aliquot at least 0.5ml of cell suspension into a sterile leak-proof container.
Ideal time to take specimen  
Labelling requirements click here
How to take the specimen  
Transport to the laboratory Bloods should arrive at the laboratory no later than 16:00 hours.
The integrity of samples may be compromised if they are not received in the laboratory on the same day as they are taken. If transport is delayed then store at +4°C
Test Times Serology: Daily
HIV-1 RNA: daily (weekdays)
HIV Genotype: Weekly
Turnround Serology: 5 working days
HIV-1 RNA: 3 working days
HIV Genotype: 2 weeks
Additional information Please complete as much details as possible on our dedicated HIV resistance forms
when requesting HIV resistance or tropism tests.
click here
Additional Examination Requests click here


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