|Investigation||Microscopy - Cell count and Gram stain|
Culture & sensitivity
|Inform lab before sending||Yes|
|Ideal time to take specimen||Ideally prior to antimicrobial therapy - although treatment should not be withheld pending investigation|
CSF Collection - |
1 x yellow-top fluoride EDTA tube (numbered 2)
2 x 28ml sterile universal container (numbered 1 & 3)
1 x grey top fluoride EDTA tube (for blood glucose)
1 x Clinical Chemistry (GREEN),
1 x Medical Microbiology (BLUE)
Specimen container must have:
|How to take specimen & Volume required||
Specimens must be collected sequentially.|
1. If Virology investigations are required then it necessary to send an additional sample. Approx 1ml in sterile universal container marked 'for Virology'.
|Transport to the laboratory||
CSF samples 1 & 3 (and any sample for Virology) to be placed in
Medical Microbiology specimen bag (with completed request form) and
dispatched immediately via the pneumatic tube system.
CSF samples 2 and blood sample to be placed in Clinical Chemistry specimen bag (with completed request form) then this specimen bag MUST be delivered by hand to Clinical Chemistry as soon as possible (within 30 minutes).
|Turnround||Results within 2 hours of receipt|
|Reference range - normal||
Relevant clinical information including a brief description of symptoms; possibility of TB, presence of known virus infections
should be included on the request form to ensure appropriate investigations are performed.
See also under Meningitis.
For information on xanthochromia analysis click here.
|Specimen retention||Please ensure that any requests for additional investigations on the specimen are made within 7 days from the date of the original request|